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Southern blot confirmed integration of copies of the nptII gene into the rose genome in the tested lines. Four transgenic lines were obtained which were morphologically true-to-type and indistinguishable from Wt shoots while they were in in vitro cultures.
Adventitious root induction was more difficult in transgenic shoots compared to the Wt shoots, however, one of the transgenic lines line 6 was rooted and subsequently analyzed phenotypically.
The ipt expression levels were determined in this line after exposure to exogenous ethylene 3. Darkness resulted in twofold up-regulation of ipt expression, whereas darkness combined with ethylene caused eightfold up-regulation in line 6 compared to Wt plants.
The transgenic line had significantly higher content of chlorophyll at the end of the treatment period compared to Wt plants. Directory of Open Access Journals Sweden Lorena M Salto Full Text Available An urgent need exists for graduate rercky ja paynes professional schools to establish evidence-based STEM science, technology, engineering, and math pipeline programs to increase the diversity of the biomedical workforce.
An untapped yet promising pool of willing participants are capable high school students that have a strong STEM interest but may lack the skills and the guided mentoring needed to succeed in competitive STEM fields. The primary focus of our summer research experience SRE is to enhance the research self-efficacy of the participants by actively involving them in a research project and by providing the students with personalized mentoring and targeted career development activities, including education on health disparities.
The results of our study show that our SRE influenced terminal degree intent and increased participant willingness to incorporate research rercky ja paynes future careers for both the HS and the UG groups. The quantitative data shows that both the HS and the UG participants reported large, statistically significant gains in self-assessed research skills and research self-efficacy.
Both participant groups identified the hands-on research and the mentor experience as the most valuable aspects of our SRE and reported rercky ja paynes science skills, increased confidence in science ability and increased motivation and affirmation to pursue a science career.